Exopolysaccharides Production by Cultivating a Bacterial Isolate from the Hypersaline Environment of Salar de Uyuni (Bolivia) in Pretreatment Liquids of Steam-Exploded Quinoa Stalks and Enzymatic Hydrolysates of Curupaú Sawdust

The halotolerant bacterial strain BU-4, isolated from a hypersaline environment, was identified as an exopolysaccharide (EPS) producer. Pretreatment liquids of steam-exploded quinoa stalks and enzymatic hydrolysates Curupaú sawdust were evaluated carbon sources for EPS production with the BU-4 strain, produced characterized using FTIR, TGA, SEM. Cultivation performed at 30 °C 48 h, cells separated culture broth by centrifugation. cell pellets ethanol precipitation, purified trichloroacetic acid treatment, followed centrifugation, dialysis, freeze-drying. stalks- sawdust-based substrates 2.73 0.89 g L?1, respectively, while 2.34 L?1 when cultivation on glucose. FTIR analysis revealed signals typical polysaccharides, well ester carbonyl groups sulfate groups. High thermal stability, water retention capacity gel-forming ability inferred SEM TGA. capability isolate producing pretreatment demonstrated, characterization their broad application potential. study shows way value-added products waste materials bacterium unique Bolivian ecosystem.